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2026 Vol.30, Issue 2

Original Article

Physicochemical and Histomorphometric Comparison of Deproteinized Bovine Bone Graft Materials
Hyun Wook An1†
,
Jin-Young Park2†
,
Seon Yeong Kim3
,
Sang Min Lee3
,
Young In Choi3
,
SuJeong Kim4
,
Yuri Kim56
,
Hyun-Chang Lim7
,
Young Woo Song8*
1Director, R&D Center, Megagen Implant Co., Ltd., Daegu, South Korea
2Assistant Professor, Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry, Seoul, South Korea
3Researcher, R&D Center, Megagen Implant Co., Ltd., Deagu, South Korea
4Researcher, Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry, Seoul, South Korea
5Lecturer, Department of Dental Hygiene, Gangneung Yeongdong University, Gangneung, South Korea
6Researcher, Department of Periodontology, Veterans Health Service Medical Center, Seoul, South Korea
7Associate Professor, Department of Periodontology, Kyung Hee University, College of Dentistry, Periodontal- Implant Clinical Research Institute, Kyung Hee University Medical Center, Seoul, South Korea
8Director, Department of Periodontology, Veterans Health Service Medical Center, Seoul, South Korea
*Corresponding Author
†These authors equally contributed to this work.
30 June 2026. pp. 61-85
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Abstract

Purpose: This study compared the physicochemical properties, in vitro biocompatibility, and in vivo bone regeneration of two bovine-derived xenogeneic bone substitutes: Bio-Oss and Mega-Oss Bovine Original.

Materials and Methods: Physicochemical characterization included SEM, XRD, TEM, FT-IR, thermogravimetric analysis, mercury intrusion porosimetry, and BET analysis. Residual organic components were quantified, and cytotoxicity was assessed using an ISO 10993-5-compliant L-929 MTT assay. In vivo evaluation used a rabbit calvarial defect model (n = 6). Four 6-mm defects per animal were filled with either material and evaluated at 4 and 8 weeks using micro-CT (BV/TV, BMD) and histomorphometric analysis.

Results: Both materials exhibited similar physicochemical characteristics, including porous microstructures, low-crystalline hydroxyapatite, and comparable Ca/P ratios and crystallite sizes. Mega-Oss Bovine Original exhibited higher porosity and a greater specific surface area than Bio-Oss. Residual lipid and protein contents were minimal in both materials, and no cytotoxic effects were observed. In the rabbit calvarial defect model, both groups demonstrated favorable tissue compatibility with minimal inflammatory responses. Micro-CT and histomorphometric analyses revealed comparable bone regeneration outcomes between the two groups at both 4 and 8 weeks, with no statistically significant differences in bone volume fraction, bone mineral density, newly formed bone area, or residual graft area (p > .05).

Conclusion: Mega-Oss Bovine Original demonstrated physicochemical characteristics, biocompatibility, and bone regeneration performance comparable to those of Bio-Oss. These findings suggest that Mega-Oss Bovine Original may serve as a suitable bovine-derived xenogeneic bone substitute for bone regeneration applications.

Keywords
Deproteinized bovine bone mineral
Histomorphometric analysis
In vitro experiment
In vivo experiment
Physicochemical analysis
References
1

Giannoudis PV, Dinopoulos H, Tsiridis E. Bone substitutes: an update. Injury 2005;36:S20-7.

10.1016/j.injury.2005.07.029
2

Barnett JA, Mellonig JT, Gray JL, Towle HJ. Comparison of freeze-dried bone allograft and porous hydroxyapatite in human periodontal defects. J Periodontol 1989;60:647-57.

10.1902/jop.1989.60.5.231
3

Mellonig JT. Freeze-dried bone allografts in periodontal reconstructive surgery. Dent Clin North Am 1991;35:505-20.

10.1016/S0011-8532(22)00854-0
4

Schllohorn RG, McClain PK. Combined osseous composite grafting, root conditioning and guided tissue regeneration. Int J Periodontics Restorative Dent 1988;8:8-31.

3075201
5

Gross J. Bone grafting material for dental application: A practical guide. Compend Contin Educ Dent 1997;18:1013-38.

9533311
6

Slotte C, Lundgren D. Augmentation of calvarial tissue using non-permeable silicone domes and bovine bone mineral. An experimental study in the rat. Clin Oral Implants Res 1999;10:468-76.

10.1034/j.1600-0501.1999.100605.x
7

de Bruijn JD, Klein CP, de Groot K, van Blitterswijk CA. The ultrastructure of the bone-hydroxyapatite interface in vitro. J Biomed Mater Res 1992;26:1365-82.

10.1002/jbm.820261008
8

Portsmouth RL, Gladden LF. Mercury porosimetry as a probe of pore connectivity. Chem Eng Res Des 1992;70:63-70.

9

Lowry OH, Rosbrough NJ, Farr AL, Randall RT. Protein measurement with the folin phenol reagent. J Biol Chem 1951;193:265-73.

10.1016/S0021-9258(19)52451-6
10

Brooks SPJ, Lampi BJ, Sarwar G, Botting HG. A comparison of methods for determining total body protein. Anal Biochem 1995;226:26-30.

10.1006/abio.1995.1186
11

Varley H. Practical clinical biochemistry. London: Heinemann Medical Books; 1965.

12

Kirik SD, Solovyov LA, Blokhin AI, Yakimov IS. Structures of [Pd(NH3)2X2] and its chemical transformation in the solid state. Acta Crystallogr B 2000;56:419-25.

10.1107/S0108768100000252
13

Lee BJ, Kim SK. Research trend of calcium materials and development of water-soluble natural calcium agents with phosphorylated peptide from fish bone. Food Ind Nutr 2005;10:40-5.

14

Rosen VB, Hobbs LW, Spector M. The ultrastructure of anorganic bovine bone and selected synthetic hyroxyapatites used as bone graft substitute materials. Biomaterials 2002;23:921-8.

10.1016/S0142-9612(01)00204-6
15

Londoño-Restrepo SM, Zubieta-Otero LF, Jeronimo-Cruz R, Mondragon MA, Rodriguez-García ME. Effect of the crystal size on the infrared and Raman spectra of bio hydroxyapatite of human, bovine, and porcine bones. J Raman Spectrosc 2019;50:1120-9.

10.1002/jrs.5614
16

Figueiredo MM, Gamelas JA, Martins AG. Characterization of bone and bone-based graft materials using FTIR spectroscopy. In : Theophanides T, editor. Infrared spectroscopy: life and biomedical sciences. London: InTech; 2012. p. 315-38.

10.5772/36379
17

Seo NR, Jang HJ, Lee SH, Kim B, Han DW. Comparative evaluation of bovine- and porcine-derived xenografts in rabbit calvarial and canine mandibular dehiscence models. Dent J (Basel) 2026;14:218.

10.3390/dj1404021842041671PMC13115451
18

Abdelmoneim D, Coates DE, Schmidlin P, Botter S, Li KC, Porter GC, et al. In vivo healing of low temperature deproteinized bovine bone xenograft in a rabbit cranial model. J Biomed Mater Res A 2024;112:1436-50.

10.1002/jbm.a.37693
19

Sohn JY, Park JC, Um YJ, Jung UW, Kim CS, Cho KS, et al. Spontaneous healing capacity of rabbit cranial defects of various sizes. J Periodontal Implant Sci 2010;40:180-7.

10.5051/jpis.2010.40.4.18020827327PMC2931306
20

Thorwarth M, Wehrhan F, Srour S, Schultze-Mosgau S, Felszeghy E, Bader RD, et al. Evaluation of substitutes for bone: Comparison of microradiographic and histological assessments. Br J Oral Maxillofac Surg 2007;45:1-47.

10.1016/j.bjoms.2006.03.013
21

Zizzari VL, Zara S, Tetè G, Vinci R, Gherlone E, Cataldi A. Biologic and clinical aspects of integration of different bone substitutes in oral surgery: A literature review. Oral Surg Oral Med Oral Pathol Oral Radiol. 2016;122:392-402.

10.1016/j.oooo.2016.04.010
22

Troeltzsch M, Troeltzsch M, Kauffmann P, Gruber R, Brockmeyer P, Moser N, et al. Clinical efficacy of grafting materials in alveolar ridge augmentation: A systematic review. J Craniomaxillofac Surg 2016;44:1618-29.

10.1016/j.jcms.2016.07.028
23

Thaller SR, Hoyt J, Dart A, Borjeson K, Tesluk H. Repair of experimental calvarial defects with bio-oss particles and collagen sponges in a rabbit model. J Craniofac Surg 1994;5:242-6.

10.1097/00001665-199409000-00009
24

Werner J, Linner-Krčmar B, Friess W, Greil P. Mechanical properties and in vitro cell compatibility of hydroxyapatite ceramics with graded pore structure. Biomaterials 2002;23:4285-94.

10.1016/S0142-9612(02)00191-6
25

Traini T, Valentini P, Iezzi G, Piatelli A. A histologic and histomorphometric evaluation of anorganic bovine bone retrieved 9 years after sinus augmentation procedure. J Periodontol 2007;78:955-61.

10.1902/jop.2007.060308
26

Traini T, Degidi M, Sammons R, Stanley P, Piatelli A. Histologic and elemental microanalytical study of anorganic bovine bone substitution following sinus floor augmentation in humans. J Periodontol 2008;79:1232-40.

10.1902/jop.2008.070504
Information
  • Publisher :The Korean Academy of Oral & Maxillofacial Implantology
  • Publisher(Ko) :대한구강악안면임플란트학회
  • Journal Title :Journal of implantology and applied sciences
  • Journal Title(Ko) :대한구강악안면임플란트학회지
  • Volume : 30
  • No :2
  • Pages :61-85
  • Received Date : 2026-05-21
  • Revised Date : 2026-06-23
  • Accepted Date : 2026-06-24
  • DOI :https://doi.org/10.32542/implantology.2026006
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