Bone Regeneration Using Collagenated Synthetic Bone Graft Attached to Collagen Membrane with 3, 4-dihydroxy-L-phenylalanine (DOPA)

Inpyo Hong; Renchindorj Dolgorsuren; Su-Hee Jeon; Young Woo Song; Jae-Kook Cha; Jung-Seok Lee; Jeong-Won Paik; Seong-Ho Choi

doi:10.32542/implantology.2021002

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2021 Vol.25, Issue 1 Preview Page Next Page

Original Article

Bone Regeneration Using Collagenated Synthetic Bone Graft Attached to Collagen Membrane with 3,4-dihydroxy-L-phenylalanine (DOPA)

31 March 2021. pp. 10-21

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Abstract

Purpose: The stability of graft material and membrane is crucial for successful guided bone regeneration. As 3,4-dihydroxy-L-phenylalanine (DOPA) is known to contribute to the adhesiveness of natural mussel adhesive proteins, the aim of this study was to evaluate the biocompatibility and bone regeneration using collagenated synthetic bone graft attached to the collagen membrane with DOPA in the rabbit calvaria model.

Materials and Methods: Four circular bone defects of 8 mm diameter were created in the rabbit calvarium. Each defect in the calvarium was randomly allocated to 1) sham control group, 2) bone graft using a particle-type synthetic bone substitute (OP) group, 3) bone graft using collagenated synthetic bone block (OC) group, or 4) bone graft using the collagenated synthetic bone graft attached to collagen membrane with DOPA (OC-DOPA) group. A total of 10 rabbits were sacrifced at either 2 or 8 weeks post-surgery.

Results: During histometric analysis, new bone formation in the OC-DOPA group was signifcantly greater than that in sham control at 8 weeks. On the contrary, there was no statistically signifcant difference in new bone area (NBA) between OC-DOPA and OC. Histologic analysis revealed infltrations of infammatory cells in the OP, OC, and OC-DOPA groups at 2 weeks. At 8 weeks, there were no remarkable infammatory cells in the OP and OC groups. However, multinucleated giant cells (MNGC) were observed till 8 weeks in the OC-DOPA group.

Conclusion: The collagenated synthetic bone graft attached to the collagen membrane using DOPA showed its potential for guided bone regeneration. However, MNGC were observed until the late healing period.

Keywords

Biphasic calcium phosphate

Collagen

4-dihydroxy-L-phenylalanine

Guided bone regeneration

Multinucleated giant cell

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Information

Publisher :The Korean Academy of Oral & Maxillofacial Implantology
Publisher(Ko) :대한구강악안면임플란트학회
Journal Title :Implantology
Journal Title(Ko) :대한구강악안면임플란트학회지
Volume : 25
No :1
Pages :10-21
Received Date : 2020-11-03
Revised Date : 2020-12-24
Accepted Date : 2021-01-18
DOI :https://doi.org/10.32542/implantology.2021002

Journal of implantology and applied sciences ISSN:2765-7833(Print) 2765-7841(Online) 대한구강악안면임플란트학회지

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