Journal of implantology and applied sciences ISSN:2765-7833(Print) 2765-7841(Online) 대한구강악안면임플란트학회지

Several materials and surface modification methods have been introduced to improve bone response onto the surface ofimplant fixtures to reduce healing time and improve osseointegration rate for implant therapy. The purpose of this studywas to evaluate the biological response of osteoblast-like cells to (between) pure titanium and zirconia composite ceramic(Zc) which has promising physical properties. Bone cells were cultured on the disc specimens and analyzed by the biochemistry, cell and molecular biology techniques tocompare sequential cell response (cell adhesion, proliferation, and differentiation) to zirconia composite ceramic and puretitanium (Ti). RT-PCR assay for the extracellular matrix (ECM) protein and SEM [evaluation of cell adhesion to Zc and Ti],RT-PCR assay for Cell Cycle Analysis (Cyclin D1) and MTS assay [evaluation of cell proliferation to Zc and Ti], ALP activitymeasurement [evaluation of cell differentiation to Zc and Ti] were used for this study.The results of this study were as follows: 1. There was no significant difference in the mRNA expression of osteonectin among control and the groups. The mRNAexpression of integrin β1 of group A,B,C (Zc) was similar to, or increased slightly more than that of control and group D,E,F (Ti). 2. SEM picture showed well-spread irregular osteoblasts on Zc. 3. The mRNA expression of cyclin D1 of group A,B,C (Zc) was similar to, or increased slightly more than that of controland group D,E,F (Ti). 4. In MTS assay, optical density (O.D.) of Zc increased slightly more than that of Ti as the culture time increased. 5. In ALP assay, control % of O.D. of Zc increased slightly more than that of Ti as the culture time increased.Within the limitations of this study, the following conclusions were drawn: Zirconia composite ceramic showed at least equivalent or slightly better biological response of osteoblast-like cells,compared with pure titanium.