Journal of implantology and applied sciences ISSN:2765-7833(Print) 2765-7841(Online) 대한구강악안면임플란트학회지

The newly developed high-strength ceramics such as zirconia may offer some advantages over the conventional bioinert materials. Especially, zirconia-alumina composite has excellent physical properties, this might be an alternative material to titanium for dental implant fabrication. This study evaluated the bone cell responses on zirconia-alumina composite discs with different roughness. A total of 18 zirconia-alumina composite disc (diameter: 19mm and thickness: 1.5mm) were prepared and divided into two groups. The discs of group 1 (n=9) had smooth surface and group2 (n=9) had rough surface(sandblasting with 150µm alumina glass particles). The cell attachment, cell proliferation, cell differentiation on the specimen were evaluated by the Real-Time Polymerase Chain Reaction(RT-PCR), Methylthiazole Sulfate(MTS) analysis, Alkaline Phosphatase(ALP) activity, and scanning electron microscopy (SEM) using osteoblast-like cell (HOS) respectively. According to result of Elisa analyses, MTS, ALP activity and SEM investigation, there was no significant difference in cell response between two different surface conditions. The analysis of RT-PCR showed that the amount of Cyclin D1(mRNA expression) was not statistically significant different between two groups after 24 hours as well, however markedly decreased in the smooth surface after 72 hours. These results suggested that, in general, the effect of the surface condition (smooth vs. rough) was not significantly affected on osteoblast like cell response to the zirconia-alumina composite in vitro iological assessment.