Journal of implantology and applied sciences ISSN:2765-7833(Print) 2765-7841(Online) 대한구강악안면임플란트학회지

Recently, new procedures using various growth factors such as PDGF, IGF-I, TGF-β have been challenged for the regeneration of intraoral bone defects. Platelet is a major source of these factors promoting new bone formation via mitogenesis and chemotaxis. The aim of this study was to investigate the effect of the autogenous platelet-rich plasma (PRP) on new bone formation around dental implants with bone defects. Total 48 titanium implants were inserted in both femurs of 24 New Zealand white rabbits following the preparation of artificial bone defects of 3.0 mm in depth and 1.85 mm of gap around the implants. All defect sites were divided into three groups and treated respectively : control, Bio-Oss(anorganic bovine bone) group, PRP+Bio-Oss group(mixtures of PRP and Bio-Oss). 4 and 8 weeks later, animals were sacrificed and undecalcified sections were prepared for histomorphometric analysis and fluorochrome labeling study. The bone-to-implant contacts of PRP+Bio-Oss groups (21.4%, 34.8%) and Bio-Oss groups (17.8%, 27.0%) were much greater than those of controls (4.6%, 13.9%) in both 4-and 8-week periods, and similar results for bone area. And more bone formation was observed in 8-weeks than in 4-weeks for all groups (P<.05). Bone regenerations in PRP+Bio-Oss groups were greater than in Bio-Oss groups for both periods, but with little statistical significances. Histologically, Bio-Oss particles were well-incorporated into newly formed bone. In addition, intense fluorochrome labelings were observed around the implants and the graft materials in PRP+Bio-Oss group indicating active bone formation and remodeling. These results suggest that the new combination procedure using Bio-Oss and autogenous platelet-rich plasma may be helpful in reinforcing the bone regeneration process.