Article | 12. 2016 Vol. 20, Issue. 4
The Effect of Resorbable Hydroxyapatite-coated PCL/ CaO-SiO2Micro/nanofibrous Membrane Fabricated by Electrospinning on Guided bone Regeneration in Rabbit Calvarial Defects






2016.12. 172:186


PDF XML




: The aim of the present study was to evaluate the effects on bone regeneration of the resorbable hydroxyapatite-coated micro/nanofibrous membranes fabricated from poly(ε-caprolactone) (PCL) and bioactive CaO-SiO2 by electrospinning in comparison with commercially available non-resorbable membranes and resorbable collagen membranes through the experiments of rabbit calvarial bone defects.

: Guided bone regeneration (GBR) surgery using non-resorbable membranes (Gore-tex®; Group NR, n=8), resorbable collagen membranes (Ossix®; Group RC, n=8) and hydroxyapatitecoated PCL/ CaO-SiO2 micro/nanofibrous membranes (Group HA, n=6) was conducted on calvarial bone defects of twenty two healthy New Zealand White male rabbits. The surface morphologies of the membranes in three groups were investigated by scanning electron microscopy prior to surgery. Histological and histomorphometric analyses were also performed at 2 and 4 weeks after surgery to evaluate the effect on bone regeneration and to assess the osteoconductivity in vivo.

: Hydroxyapatites coating was confirmed on the membranes made of PCL and CaO-SiO2 gel fibers in group HA, whereas it was not identified in the other groups. Histological analyses revealed that large amount of newly formed bone existed over the defect area in all groups. A remarkable feature was that new bone formation was under way directly beneath the membrane or as closely incorporated pattern into the membrane structure indicating osteoconductivity and vigorous bone formation in group HA. Histomorphometric results revealed that there was a tendency for larger amount of newly formed bone to exist at 2 weeks after surgery in group HA compared to other groups, however, it did not reach statistical significance.

: The resorbable hydroxyapatite-coated micro/nanofibrous membranes fabricated from electrospun PCL and bioactive CaO-SiO2 have high osteoconductivity and promoting effect of new bone formation in GBR of rabbit calvarial defects in the early stage of bone healing.



1. Lekholm U, Adell R, Lindhe J, et al. Marginal tissue reactions at osseointegrated titanium fixtures. (II) A cross-sectional retrospective study. Int J Oral Maxillofac Surg. 1986; 15: 53-61.  

2. McAllister BS, Haghighat K. Bone augmentation techniques. J Periodontol. 2007; 78: 377-396.  

3. Liu J, Kerns DG. Mechanisms of guided bone regeneration: a review. Open Dent J. 2014; 8: 56-65.  

4. Bottino MC, Thomas V, Schmidt G, et al. Recent advances in the development of GTR/GBR membranes for periodontal regeneration--a materials perspective. Dent Mater. 2012; 28: 703-721.  

5. Abou Neel EA, Bozec L, Knowles JC, et al. Collagen--emerging collagen based therapies hit the patient. Adv Drug Deliv Rev. 2013; 65: 429-456.  

6. Gentile P, Chiono V, Tonda-Turo C, et al. Polymeric membranes for guided bone regeneration. Biotechnol J. 2011; 6: 1187-1197.  

7. Mo XM, Xu CY, Kotaki M, et al. Electrospun P(LLA-CL) nanofiber: a biomimetic extracellular matrix for smooth muscle cell and endothelial cell proliferation. Biomaterials. 2004; 25: 1883-1890.  

8. Xu C, Inai R, Kotaki M, et al. Electrospun nanofiber fabrication as synthetic extracellular matrix and its potential for vascular tissue engineering. Tissue Eng. 2004; 10: 1160-1168.  

9. Kwon IK, Kidoaki S, Matsuda T. Electrospun nano- to microfiber fabrics made of biodegradable copolyesters: structural characteristics, mechanical properties and cell adhesion potential. Biomaterials 2005; 26: 3929-3939.  

10. Luu YK, Kim K, Hsiao BS, et al. Development of a nanostructured DNA delivery scaffold via electrospinning of PLGA and PLA-PEG block copolymers. J Control Release. 2003; 89: 341-353.  

11. Seol YJ, Kim KH, Kim IA, et al. Osteoconductive and degradable electrospun nonwoven poly (epsilon-caprolactone)/CaO-SiO gel composite fabric. J Biomed Mater Res A. 2010; 94: 649-659.  

12. Wiltfang J, Merten HA, Peters JH. Comparative study of guided bone regeneration using absorbable and permanent barrier membranes: a histologic report. Int J Oral Maxillofac Implants. 1998; 13: 416-421.  

13. Kasaj A, Reichert C, Gotz H, et al. In vitro evaluation of various bioabsorbable and nonresorbable barrier membranes for guided tissue regeneration. Head Face Med. 2008; 4: 22.  

14. Gielkens PF, Schortinghuis J, de Jong JR, et al. Vivosorb, Bio-Gide, and Gore-Tex as barrier membranes in rat mandibular defects: an evaluation by microradiography and micro-CT. Clin Oral Implants Res. 2008; 19: 516-521.  

15. Ignjatovic N, Wu V, Ajdukovic Z, et al. Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues. Mater Sci Eng C Mater Biol Appl. 2016; 60: 357-364.  

16. Huhtala A, Pohjonen T, Salminen L, et al. In vitro biocompatibility of degradable biopolymers in cell line cultures from various ocular tissues: extraction studies. J Mater Sci Mater Med. 2008; 19: 645-649.  

17. Owen GR, Jackson JK, Chehroudi B, et al. An in vitro study of plasticized poly(lactic-co-glycolic acid) films as possible guided tissue regeneration membranes: material properties and drug release kinetics. J Biomed Mater Res A. 2010; 95: 857-869.  

18. Kang YM, Kim KH, Seol YJ, et al. Evaluations of osteogenic and osteoconductive properties of a non-woven silica gel fabric made by the electrospinning method. Acta Biomater. 2009; 5: 462-469.  

19. Kim HW, Kim HE. Nanofiber generation of hydroxyapatite and fluor-hydroxyapatite bioceramics. J Biomed Mater Res B Appl Biomater. 2006; 77: 323-328.  

20. Lao L, Wang Y, Zhu Y, et al. Poly(lactide-co-glycolide)/hydroxyapatite nanofibrous scaffolds fabricated by electrospinning for bone tissue engineering. J Mater Sci Mater Med. 2011; 22: 1873-1884.  

21. Rhee SH, Choi JY, Kim HM. Preparation of a bioactive and degradable poly(epsilon-caprolactone)/ silica hybrid through a sol-gel method. Biomaterials. 2002; 23: 4915-4921.  

22. Sun H, Mei L, Song C, et al. The in vivo degradation, absorption and excretion of PCL-based implant. Biomaterials. 2006; 27: 1735-1740.  

23. Cho SB, Miyaji F, Kokubo T, et al. Apatite-forming ability of silicate ion dissolved from silica gels. J Biomed Mater Res. 1996; 32: 375-381.  

24. de Santana RB, de Mattos CM, Francischone CE, et al. Superficial topography and porosity of an absorbable barrier membrane impacts soft tissue response in guided bone regeneration. J Periodontol. 2010; 81: 926-933.  

25. Chvapil M, Holusa R, Kliment K, et al. Some chemical and biological characteristics of a new collagen-polymer compound material. J Biomed Mater Res. 1969; 3: 315-332.